产品说明
一般描述
Desmocollin-2, also known as Desmocollin-3 or Desmosomal glycoprotein II, or Desmosomal glycoprotein III, and encoded by the gene DSC2/CDHF2/DSC3, is a component of intercellular desmosome junctions. Desmocollin-2 is involved in the interaction of plaque proteins and intermediate filaments that mediate cell-cell adhesion and interacts with DSP, PKP2 and JUP proteins. Additionally, Desmocollin-2 is important for epidermal cell positioning and stratification because it mediates the differential adhesiveness between cells that express different isoforms. Desmocollin-2 is localized to the cell membrane at cell junctions and desmosomes. Desmocollin-2 is expressed in epithelia, myocardium and lymph nodes. Defects in Desmocollin-2 may be associated with Familial arrhythmogenic right ventricular dysplasia 11 (ARVD11) a congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.
免疫原
MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2.
Epitope: This antibody recognizes the extracellular domain of Desmocollin-2.
应用
Research Category
Cell Structure
This Anti-Desmocollin-2 Antibody, clone 7G6 is validated for use in western blotting, IP, IHC & ICC for the detection of Desmocollin-2.
Immuonoprecipitation Anlaysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells transfected with C-terminal deletions of plakoglobin retaining 707 amino acids (Wahl, J. K. 3rd, et al. (1996). J Cell Sci. 109(Pt 5):1143-1154.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in Primary human Keratinocytes (Franke, WW., et al (2006). Eur. J. Cell Biol. 85(2); 69-82.)
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Desmocollin-2 in A431 cells (Wahl, J. K. 3rd. (2005). J Cell Biochem. 96(2):390-403.).
Research Sub Category
ECM Proteins
质量
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Desmocollin-2 in 10 µg of A431 cell lysate.
目标描述
~110 kDa observed
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | 7G6, monoclonal |
species reactivity | human |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG1κ |
NCBI登记号 | NP_004940 |
UniProt登记号 | Q02487 |
运输 | wet ice |
Gene Information | human ... DSC3(1825) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |