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Anti-DDX6-Alexa488 antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200879
规格纯度
clone DDX6-34, purified from hybridoma cell culture
参考价格
1801.29 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Probable ATP-dependent RNA helicase DDX6 also known as DEAD box protein 6 or Oncogene RCK/p54, is a conserved member of the DEAD-box RNA helicase family.DDX6 plays a central role in P-body assembly in mammals and its depletion prevents P-body assembly leading to P-bodies disappearance.1

特异性

Monoclonal Anti-DDX6 antibody specifically recognizes DDX6 from human, mouse, monkey, canine and bovine origin.

应用

The antibody may be used in Immunofluorescence techniques to detection of DDX6.

生化/生理作用

DDX6 coordinates RNA and ATP helicase binding activities and binds along the mRNA. DDX6 sequence specific binding at mRNA 5′ end, activates recruitment of the decapping complex, thus coupling translational repression and mRNA degradation.1-2 DDX6 is considered to be a P-bodies marker due to its mediating role between translational repression and P-bodies formation and toits interaction with Ago2 and the RISC complex that also mediates translational silencing by miRNAs.1-4

Viruses like Hepatitis C virus (HCV) or West Nile virus (WNV) utilize the host cell DDX6 to promote virus infection by recruiting it to the viral replication sites where it is involved in stabilization of the viral genome and the shift between translation and replication.2,5-6
DDX6 was reported to be overexpressed in colorectal adenocarcinomas, gastric cancer and in most malignant cell lines.7-9 It was suggested that DDX6 acted as an upstream cancerous enhancer through RNA-binding of HER2 and FGFR2 mRNAs that positively regulates post-transcriptional processes in cancer cells.7

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.

法律信息

ALEXA FLUOR is a registered trademark of Life Technologies

免责声明

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

产品性质

质量水平200
偶联物ALEXA FLUOR® 488 conjugate
抗体形式purified from hybridoma cell culture
克隆DDX6-34
形式liquid
species reactivitymouse, monkey, human, bovine, canine
浓度1.5-2 mg/mL
同位素/亚型IgG1
UniProt登记号P26196
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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