产品说明
一般描述
DBF4, also known as Protein DBF4 homolog A, Activator of S phase kinase, also known as Chiffon homolog A or DBF4-type zinc finger containing protein 1, and encoded by the gene DBF4/ASK/DBF4A/ZDBF1, is the regulatory subunit for the cell cycle kinase CDC7. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at ′Ser-40′ and ′Ser-53′ and then is involved in regulating the initiation of DNA replication during cell cycle. . DBF4 Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2, ORC4 and ORC6 proteins. DBF4 is localized to the nucleus. DBF4 is expressed in expressed in many cells particularly high in testis, thymus and most cancer cells.
免疫原
GST-tagged recombinant protein corresponding to human DBF4 near the C-terminus.
Epitope: Near C-terminus
应用
Research Category
Epigenetics & Nuclear Function
Anti-DBF4 antibody, clone 7H8.1 detects level of DBF4 & has been published & validated for use in DBF4.
Research Sub Category
Chromatin Biology
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected DBF4 in 200 µg of human thymus tissue lysate.
质量
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected DBF4 in 200 µg of HepG2 cell lysate.
目标描述
~77 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 7H8.1, monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NP_006707 |
| UniProt登记号 | Q9UBU7 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
