Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1

产品说明

一般描述

Cullin-4A (UniProt Q13619; also known as CUL-4A) is encoded by the CUL4A gene (Gene ID 8451) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. Cullin-4 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The human genome encodes 8 cullin proteins (CUL 1, 2, 3, 4A, 4B, 5, 7 and 9). The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). CUL4A interacts with the substrate-binding adaptor DDB1 (DNA damage binding protein 1) via its arc-shaped helical N-terminal domain, and binds the small RING finger protein ROC1 (regulator of cullins 1) via its globular C-terminal domain. Despite the 83% sequence identity and seemingly functional redundancy between CUL4A and CUL4B, much more focus is placed on CUL4A in oncogenesis research.

特异性

Clone 2A2.1 is expected to react with both spliced isoforms of human cullin-4A

免疫原

KLH-conjugated linear peptide corresponding to human Cullin-4A/CUL4A near the C-terminus.
Epitope: Near C-terminus

应用

Research Sub Category
Apoptosis - Additional
This Anti-Cullin-4A/CUL4A Antibody, clone 2A2.1 is validated for use in Western Blotting for the detection of Cullin-4A/CUL4A.
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blotting in human melanoma SK-MEL-28 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-4A/CUL4A in 10 µg of human melanoma SK-MEL-28 cell lysate.

目标描述

~88 kDa observed

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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