产品说明
一般描述
The extracellular matrix (ECM) found in the extracellular environment of all tissues and organs, provides the physical microenvironment for cells and a substrate for cell anchorage. It serves as a tissue scaffold and is a dynamic structure whose organization and composition modulate various cellular processes including cell proliferation, attachment, migration, differentiation and survival. The composition of the extracellular framework of all vertebrates is dominated by a Collagen protein family, each member with unique features suited for its function and location.
Type X collagen,also known as Collagen alpha-1(X) chain (COL10A1), is a product of hypertrophic chondrocytes. It shares a similar domain structure with type VIII collagen. In addition, both collagen types represent major components of hexagonal lattice structure, in which the collagen molecules link together by interactions involving the non-triple-helical end regions. Despite these similarities, a distinct tissue distribution has been found for these two molecules: type VIII collagen is distributed in various tissues, whereas type X is restricted to normal fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs and in adult (> 21 yr) thyroid cartilage. It is also found in bone fracture callus, osteoarthritic cartilage and chondrogenic neoplasms, and may be involved in cartilage mineralization. Type X collagen is non-fibrillar, but forms fine pericellular filaments in association with cartilage collagen. It interacts with matrix proteins, such as connexin V, chondrocalcein, collagen II and proteoglycans, as well as with Ca2+ . Mutations in this gene are associated with schmid metaphyseal chondroplasia (MCDS).
The development of antibodies against collagens has provided a powerful method for examining the distribution of these connective tissue proteins and for investigation of epithelial-mesenchymal interactions, tumorigenesis and basement membrane biology in ontogeny and epithelial differentiation.8 Antibodies that react specifically with collagen type X are useful for the study of specific differential tissue expression and the localization of collagen type X.
免疫原
Porcine collagen type X
应用
Monoclonal Anti-Collagen, Type X specifically recognizes native and denatured collagen type X. It does not recognize collagen types I, II, III, V, IX and XI. Reactivity has been observed with human, deer and porcine collagen type X. The antibody is recommended to use in various immunological techniques, including Immunoblotting (∼ 60 kDa in denatured-reduced preparation), Immunofluorescence and Immunohistochemistry.
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
其他说明
This product is for R&D use only, not for drug, household, or other uses.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | mouse |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | COL-10, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | ~60 kDa |
| species reactivity | deer, porcine, human |
| 包装 | antibody small pack of 25 μL |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoblotting: suitable immunofluorescence: 5-10 μg/mL using human osteosarcoma SaOS-2 cells immunohistochemistry: suitable |
| 同位素/亚型 | IgM |
| UniProt登记号 | Q03692 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
