产品说明
特异性
Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
免疫原
Human and bovine placental collagen type IV
应用
Research Category
Cell Structure
Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Research Sub Category
ECM Proteins
Dot and slot blotting: 1:100-1:500
ELISA: 1:1000-1:4000
Indirect immunohistochemistry (frozen sections only): 1:10-1:40
Immunocytochemistry 1:10-1:20
Optimal working dilutions must be determined by the end user.
外形
Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose® 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified
储存及稳定性
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a registered trademark of Cytiva
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | goat |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | human, mouse, bovine |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable dot blot: suitable immunocytochemistry: suitable immunohistochemistry: suitable |
适用性 | not suitable for Western blot not suitable for immunohistochemistry (Paraffin) |
NCBI登记号 | NM_001845.4 |
UniProt登记号 | P02462 |
运输 | wet ice |
Gene Information | human ... COL4A1(1282) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | nwg |
闪点(F) | Not applicable |
闪点(C) | Not applicable |