产品说明
一般描述
Mast cell chymase (EC 3.4.21.39), a member of the granzyme subfamily of the S1 family of peptidases, cleaves C-terminal to Phenylalanine, Tyrosine, Tryptophan, and Leucine residues (in order of preference). Chymase is stored in Mast cell granules within the cell and is the major secreated Mast cell protease. Secreated Mast cell chymase is believed to be involved in extracellular matrix degradation, vasoactive peptide generation, and regulation of gland secreation.
特异性
Reacts with human mast cell chymase.
免疫原
Chymase purified from human skin.
应用
Research Sub Category
Inflammation & Autoimmune Mechanisms
Dot blotting: 0.5-1.0 μg/mL.
Immunohistochemistry: 0.5-1.0 μg/mL. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue.
Not recommended for use on Western blots.
Optimal working dilutions must be determined by end user.
Anti-Chymase Antibody, clone B7 is an antibody against Chymase for use in DB, IH.
Research Category
Inflammation & Immunology
外形
Purified by protein G affinity chromatography. Liquid in PBS with 20% glycerol, pH 7.4.
Format: Purified
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt. Antibody may also be stored at -20ºC in undiluted aliquots.
分析说明
Control
POSITIVE CONTROL: lung, heart, skin, placenta.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | B7, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
浓度 | 2.4 mg/mL |
technique(s) | dot blot: suitable immunohistochemistry: suitable |
同位素/亚型 | IgG1κ |
适用性 | not suitable for Western blot |
NCBI登记号 | NM_001836.2 |
UniProt登记号 | P23946 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |