产品说明
一般描述
In enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).
特异性
This antibody recognizes cholesterol-24 hydroxylase.
免疫原
His-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.
Epitope: unknown
应用
Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.
Research Sub Category
Signaling Neuroscience
Research Category
Neuroscience
质量
Evaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.
Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.
目标描述
~ 54 kDa
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse brain lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 1A7, monoclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (based on 100% sequence homology), rabbit (based on 100% sequence homology), rat (based on 100% sequence homology) |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_006659 |
| UniProt登记号 | Q9Y6A2 |
| 运输 | wet ice |
| Gene Information | human ... CYP46A1(10858) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
