产品说明
一般描述
CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
免疫原
Linear peptide corresponding to human CHD-4.
应用
Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
质量
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.
目标描述
~220 kDa observed
外形
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 3F2/4, monoclonal |
| species reactivity | human, mouse |
| technique(s) | ChIP: suitable immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NP_001264 |
| UniProt登记号 | Q14839 |
| 运输 | wet ice |
| Gene Information | human ... CHD4(1108) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
