Anti-CDT2 Antibody

产品说明

一般描述

CDT2, also known as DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, or Retinoic acid-regulated nuclear matrix-associated protein, and encoded by the gene DTL/CDT2/CDW1/DCAF2/L2DTL/RAMP, is a component of the DCX(DTL) E3 ubiquitin ligase complex and is the substrate-specific adapter for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT2 is important because degradation of CDT1 in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDT2 is localized to the nucleus primarily but it translocates to the cytoplasm during mitosis. CDT2 is expressed in multiple tissues at varying degrees with high expression in adult placenta, testis, thymus, bone marrow and fetal all fetal tissues, and low expression in adult muscle, spleen and lymph node.

免疫原

Recombinant protein corresponding to human CDT2.

应用

Research Category
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair
Anti-CDT2 Antibody detects level of CDT2 & has been published & validated for use in CDT2.
Western Blotting Analysis: A representative lot from an independent laboratory detected CDT2 in transfected 293T cell lysates (Abbas, T., et al. (2013). Mol Cell. 49(6):1147-1158.).

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDT2 in 10 µg of A431 cell lysate.

目标描述

~85 kDa observed

外形

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息