Anti-Brevican Antibody, clone N294A/6

产品说明

一般描述

Brevican core protein (BCAN), also known as Brain-enriched hyaluronan-binding protein (BEHAB) or Chondroitin sulfate proteoglycan 7 (CSPG7), may play a role in the terminally differentiating and the adult nervous system during postnatal development. It may stabilize interactions between hyaluronan (HA) and brain proteoglycans. Two isoforms exist, with isoform 1 (~99 kDa) being highly expressed from birth through 8 years of age and down-regulated by 20 years of age to low levels that are maintained in the normal adult cortex; and isoform 2 (~72 kDa) being expressed at uniformly low levels throughout development.

免疫原

Recombinant protein corresponding to rat Brevican.

应用

Western Blot Analysis: 2 µg/mL from a representative lot detected Brevican in 10 µg of human brain tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.

Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Anti-Brevican, clone N294A/6 is an antibody targeting the Brevican protein, validated for use in WB & IHC.

质量

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.

目标描述

~140 kDa observed. The calculated molecular weight of this protein is 96 kDa A second isoform is known to exist at 69 kDa This protein may be observed at a higher molecular weight due to glycosylation. A proteolyticaly cleaved fragment of Brevican may be observed at ~50 kDa in some tissue lysates.

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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