产品说明
一般描述
ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity
特异性
The antibody reacts with the 50 kDa ATG5 protein.
Other species have not been tested.
免疫原
Linear Peptide, aa: 8-318
应用
Research Sub Category
Apoptosis - Additional
This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.
Immunoprecipitation: HeLa cell lysate
Research Category
Apoptosis & Cancer
质量
Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.
目标描述
50 kDa
联系
Replaces: MABC137
外形
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Western Blot and Immunoprecipitation: HeLa cell lysate.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 177.19, monoclonal |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| NCBI登记号 | NM_004849.2 |
| UniProt登记号 | Q9H1Y0 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
