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Anti-APP585C

品牌
Sigma-Aldrich
货号
ABN1641
规格纯度
serum, from rabbit
参考价格
3507.4 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Amyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI. APP, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. Deposition of Amyloid beta (Ab) is an early event in the pathogenesis of Alzheimer s disease (AD). Ab peptides originate from the proteolytic cleavage of the amyloid precursor protein (APP). APPs are cell surface protein that are internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. The beta-secretase cleaves APP between residues Met671 and Asp672 and yields sAPP beta and C99. Following the beta-secretase cleavage, a second cleavage occurs at the C-terminus of Ab peptide that releases Ab from C99. This cleavage occurs in the vicinity of residue 712 of the C-terminus. The gamma-secretase can cleave the C-terminal region at either Val711 or Ile713 to produce the shorter Ab peptide (Ab1-40) or the longer Ab peptide (Ab1-42). Ab1-42 occurs more frequently and forms fibrillar aggregates far more readily than the Ab1-40 peptide. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu2+ and Fe3+ to Cu+ and Fe2+, respectively. Abeta42 is a more effective reductant than beta-amyloid 40. From a physiological point of view, it functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. It is also involved in cell mobility and transcription regulation through protein-protein interactions and can promote transcription activation through binding to APBB1-KAT5.

特异性

This polyclonal antibody detects amyloid precursor protein in human Alzheimer′s disese brain. It targets an epitope within 9 amino acids in the vicintiy of APP585 in the C-terminal region.

免疫原

Epitope: extracellular domain
A linear peptide corresponding to 9 amino acids from the C-terminal region of human Amyloid beta A4 protein.

应用

Western Blotting Analysis (WB): A 1:1000 Dilution of this antibody detected Amyloid beta A4 protein in 5XFAD and 5XFAD/AEP-/- mouse brain lysate.
Western Blotting Analysis (WB): A Dilution of this antibody detected Amyloid beta A4 protein in PMID 26549211 .
Immunohistochemistry Analysis (IHC): A Dilution of this antibody detected Amyloid beta A4 protein in PMID 26549211 .
Immunohistochemistry Analysis (IHC): A 1:200 Dilution of this antibody detected Amyloid beta A4 protein in 5XFAD familial AD transgenic mouse brain.
Anti-APP585C, Cat. No. ABN1641, is a highly specific rabbit polyclonal antibody that targets Amyloid beta A4 protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience

质量

Evaluated by Immunohistochemistry in human Alzheimer′s diseased human brain tissue.

Immunohistochemistry Analysis: A 1:200 dilution of this antibody detected APP585C in Alzheimer′s diseased human brain tissue.

目标描述

10 kDa calculated.

外形

Unpurified
Rabbit polyclonal antiserum with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivitymouse, human
species reactivity (predicted by homology)canine (based on 100% sequence homology), rat (based on 100% sequence homology), porcine (based on 100% sequence homology), rabbit (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
包装antibody small pack of 25 μL
technique(s)immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG
NCBI登记号NP_000475
UniProt登记号P05067
运输ambient
Gene Informationhuman ... APP(351)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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