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Anti-Ankyrin Antibody, clone Ank016

品牌
Sigma-Aldrich
货号
MAB1683
规格纯度
clone Ank016, Chemicon®, from mouse
参考价格
6861.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Reacts with the intracellular ankyrin (216 kDa). Ankyrin consists of a single peptide of M.W. 215 kDa known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues).

免疫原

Purified human erythroid cells ankyrin

应用

Research Category
Cell Structure
Anti-Ankyrin Antibody, clone Ank016 is an antibody against Ankyrin for use in ELISA, FC, IP, WB & IC.
Western blotting at 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals.

ELISA: 1:1000

Immunoprecipitation: 1:100

Immunocytochemistry, using 2% paraformaldehyde fixation; higher fixation (i.e. 4% PFA can be problematic) 1:100; light fixation 5-10′ RT usually; permeabilize lightly with 0.1% triton X-100 as protein is intracellular.

Flow cytometry: 1:500

Immunohistochemistry: 1:100; light fixations suggested; PLP or mild zinc formalin recommended.

RIA: 1:5000

Optimal working dilutions must be determined by end user.
Research Sub Category
Cytoskeleton

外形

Purified immunoglobulin from culture supernatant. Liquid in 10 mM sodium phosphate, pH 7.5 and 150mM NaCl with 0.01% NaN3.
Format: Purified

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆Ank016, monoclonal
species reactivitymouse, canine, bovine, rabbit, pig, rat, human
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2a
NCBI登记号NM_000037.3
NM_020475.2
NM_020476.2
NM_020477.2
NM_020478.3
NM_020479.2
NM_020480.3
NM_020481.2
UniProt登记号P16157
运输dry ice
Gene Informationhuman ... ANK1(286)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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