Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5

产品说明

特异性

Reacts with the natural amyloid precursor protein A4(695).

免疫原

Epitope: A4(695)
Synthetic peptide Alz 90 [amino acids 511-608 of amyloid precursor protein pre A4(695)].

应用

This Anti-Amyloid Precursor Protein Antibody, A4(695), clone 1.D5 is validated for use in WB for the detection of Amyloid Precursor Protein.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western blot: 10-20 μg/mL

Optimal working dilutions must be determined by the end user.

Westen Blot Protocol for MAB349

1) After blotting the SDS-PAGE gel onto nitrocellulose, incubate at room temperature for one hour in 1% PBS in 0.1 mol/L PBS, pH 7.2, to block non-specific background.

2) Incubate the Western blot with MAB349 (10-20 μg/mL) at +4°C overnight shaking well.

3) Wash the Western blots 2 times in PBS-Tween 0.05% for ten minutes. Wash one time in PBS.

4) Incubate the blots with anti-Mouse IgG (Chemicon AP124) for one hour at room temperature.

5) Repeat step #3.

6) Incubate in peroxidase-anti-peroxidase [PAP] (Chemicon PAP14) according to manufacturers directions.

7) Repeat step #3.

8) Incubate in 4-chloro-1-napthol for twenty minutes at room temperature.

9) Repeat step #3.

外形

Liquid in 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide.
Format: Purified

储存及稳定性

Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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