产品说明
一般描述
Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1.
AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa).
免疫原
fusion protein corresponding to the N-terminus of human AKT1S1.
The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1.
应用
Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation.
生化/生理作用
Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
基本信息
NACRES | NA.44 |
产品性质
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | AKT1S1-3, monoclonal |
形式 | buffered aqueous solution |
分子量 | antigen ~40 kDa |
species reactivity | human |
浓度 | ~1.0 mg/mL |
technique(s) | immunoprecipitation (IP): suitable western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells. |
同位素/亚型 | IgG1 |
UniProt登记号 | Q96B36 |
运输 | dry ice |
储存温度 | −20℃ |
Gene Information | human ... AKT1S1(84335) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |