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Anti-AKT1S1 antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
SAB4200256
规格纯度
clone AKT1S1-3, purified from hybridoma cell culture
参考价格
5045.46 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1.
AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa).

免疫原

fusion protein corresponding to the N-terminus of human AKT1S1.
The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1.

应用

Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation.

生化/生理作用

Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

基本信息

NACRESNA.44

产品性质

生物来源mouse
偶联物unconjugated
抗体形式purified from hybridoma cell culture
antibody product typeprimary antibodies
克隆AKT1S1-3, monoclonal
形式buffered aqueous solution
分子量antigen ~40 kDa
species reactivityhuman
浓度~1.0 mg/mL
technique(s)immunoprecipitation (IP): suitable
western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells.
同位素/亚型IgG1
UniProt登记号Q96B36
运输dry ice
储存温度−20℃
Gene Informationhuman ... AKT1S1(84335)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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