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Anti-Acetylcholinesterase (AChE) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
SAB4200839
规格纯度
IgG fraction of antiserum, buffered aqueous solution
参考价格
2103.08 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Acetylcholinesterase (AChE) is a member of the a/ß-hydrolase-fold proteins superfamily which is composed of structurally related proteins with a great diversity in their catalytic, recognition, adhesion and chaperone functions. 3D structure studies of a/ß-hydrolase reviled a variety of folding patterns suggesting associations through oligomerization dependent functions.1 AChE is a tissue specific serine hydrolase expressed in several variants at neuromuscular junctions (NMJs)2, cholinergic brain synapses2-3 or erythrocyte (Red Blood Cells- RBC)4.

特异性

Anti-Acetylcholinesterase (AChE) antibody specifically recognizes human AChE.

免疫原

human Acetylcholinesterase, recombinant, expressed in HEK 293 cells

应用

The antibody may be used in various immunochemical techniques including Immunoblotting Immunofluorescence and ELISA. Detection of the AChE band by Immunoblotting is specifically inhibited by the immunogen.

生化/生理作用

In the synaptic cleft, AChE main function is to ensure rapid hydrolysis of acetylcholine (ACh) released from motor nerve ending into choline and acetic acid thus preventing postsynaptic muscle ACh receptors (AChR) hyperactivation and termination of the signal transmission.2-3,5AChE and Butyrylcholinesterase (BChE) inhibitory drugs or peptides are being used in several neurodegenerative diseases including Alzheimer′s disease and pathological muscle weakness such as Myasthenia Gravis. These AChE inhibitors increase synaptic ACh levels and hence improve the cholinergic function in the brain.2-3,5-6 Erythrocytes AChE (AChE-H) is a membrane encored AChE splicing variant found in RBC, it belongs to the glycosylphosphatidylinositol (GPI) protein family and carries the Yta blood group antigen. Erythrocytes AChE can be found in plasma circulating vesicles originated from aged erythrocytes, and therefore is considered as a biomarker for RBC membrane integrity. Furthermore, Erythrocytes AChE activity is also a marker for inflammation and other diseases including essential hypertension, glaucoma, ALS, neurotoxicity, and Hirschsprung′s disease.4,7-8

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

储存及稳定性

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
抗体形式IgG fraction of antiserum
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivityhuman
包装antibody small pack of 25 μL
浓度~1 mg/mL
technique(s)immunoblotting: 1:1000-1:2000 using whole extracts of Jurkat cells.
immunofluorescence: 1:1000-1:20000 using Human A431
UniProt登记号P22303
运输dry ice
储存温度−20℃
Gene Informationhuman ... ACHE(43)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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