产品说明
一般描述
Histones H3 and H4 are the predominant histones subjected to extensive covalent modifications. It is an important component of chromatin structure.
免疫原
synthetic peptide containing acetylated Lys12 of human histone H4, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H4.
应用
Anti-acetyl-Histone H4 [Ac-Lys12] antibody produced in rabbit has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
Histone H4 can be reversibly acetylated at lysine residues K5, K8, K12 and K16, and the acetylation occurs initially at K16 and then propagates in an N-terminal direction through K12, K8 and K5. Altered acetylation of histone H4 on Lys12 (H4- K12Ac) is linked to age-dependent memory impairment in mice. Histones are subjected to several covalent modifications, such as phosphorylation, methylation, acetylation and ubiquitination, that affect chromatin structure and regulate chromatin activity. Histone modifications plays an important role in cancer and disease.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~12 kDa |
| species reactivity | rat, mouse, human |
| technique(s) | immunohistochemistry: 1:100-1:200 using formalin-fixed paraffin-embedded human breast carcinoma indirect immunofluorescence: 1:250-1:500 using HeLa cells western blot: 1:500-1:1000 using NIH3T3 cell lysates |
| UniProt登记号 | Q16695 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HIST3H3(8290) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
