产品说明
一般描述
The HIST3H3 gene is mapped on the human chromosome at 1q42.13. Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein.
特异性
Anti-acetyl-Histone H3 (Ac-Lys27) specifically recognizes human and mouse (Ac-Lys27) Histone H3 (H3K27Ac).
免疫原
synthetic peptide containing acetylated Lys27 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3
应用
Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit may be used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
生化/生理作用
Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. Active chromatin is highly correlated with the hyperacetylation of histone H3 at the N-terminal tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56 by a family of enzymes known as histone acetyltransferases (HATs). Histone H3 can be modified at Lys27 either by methylation or acetylation. Histones are thought to play an important role in development and cancer.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~15 kDa |
| species reactivity | rat, human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunohistochemistry: 10-20 μg/mL indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells western blot: 1-2 μg/mL using extracts of NIH3T3 cells |
| UniProt登记号 | Q16695 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... HIST3H3(8290) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
