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AKT1 (E17K), Active

品牌
Sigma-Aldrich
货号
SRP0721
规格纯度
PRECISIO® Kinase, recombinant
参考价格
5177.2 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Useful for kinase assay.

生化/生理作用

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias (2).

重悬

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

储存及稳定性

Store in aliquots at -70℃. Avoid freeze/thaw cycles.

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

产品性质

重组expressed in baculovirus infected Sf9 cells
测定70-95% (SDS-PAGE)
形式frozen liquid
specific activity50-75 nmol/min·mg (radiometric)
分子量 ~85 kDa
浓度0.1 μg/μL
登记号NM_005163
运输dry ice
储存温度−70℃
Gene Informationhuman ... AKT1(207)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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