产品说明
一般描述
Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable.
适用性
Suitable for HDL and LDL/VLDL cholesterol detection in serum samples and for the evaluation of drugs on cholesterol metabolism.
原理
The AF HDL and LDL/VLDL Assay Kit is based on an improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to total cholesterol concentration in the sample. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorometric assays.
产品性质
| 用途 | sufficient for 100 colorimetric or fluorometric tests |
| detection method | colorimetric |
| 储存温度 | −20℃ |
安全信息
| 象形图 |     |
| 警示用语： | Danger |
| 危险声明 | H225 - H315 - H318 - H336 - H410 |
| 预防措施声明 | P210 - P233 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338 |
| 危险分类 | Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Central nervous system |
| 储存分类代码 | 3 - Flammable liquids |
| WGK | WGK 3 |
