产品说明
一般描述
Wnt-7a is expressed in placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Most Wnt proteins can signal though a mechanism called the canonical Wnt pathway, in which Wnt proteins bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of β-Catenein degradation. Intracellular accumulation of β-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors and induces the expression of numerous genes. Increased Wnt/β-Catenein signaling is associated with tumorigenesis in a diverse set of human cancers. However, Wnt-7a/Frizzled-9 signaling has been shown to act as a tumor suppressor in non-small cell lung cancers.
生化/生理作用
Wnt-7a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Recombinant human Wnt-7a is a 35.5 kDa glycoprotein containing 318 amino acids. Due to glycosylation, Wnt-7a migrates between 40-55 kDa by SDS-PAGE gel under unreduced conditions
外形
Lyophilized with no additives.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ≤0.2 mg/mL. Do not vortex. This solution can be stored at 2-8℃ for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20℃ to -80℃.
基本信息
| NACRES | NA.32 |
产品性质
| 生物来源 | human |
| 重组 | expressed in HEK 293 cells |
| 测定 | ≥80% (HPLC) ≥80% (SDS-PAGE) |
| 形式 | lyophilized |
| 效能 | 40-60 ng/mL ED50 |
| 分子量 | 35.5 kDa |
| 包装 | pkg of 15 μg |
| 杂质 | <0.1 EU/μg endotoxin, tested |
| 颜色 | white |
| UniProt登记号 | O00755 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... WNT7A(7476) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
