产品说明
应用
Tris HCl is a commonly used biological buffer. Tris/Tris HCl as a buffering system has a very broad usage in biological systems for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. The pKa of Tris is 7.77 which corresponds closely to a physiological pH of 7.36.
Tris HCl is used as the conjugate buffer component with Tris/Tromethamine/Trizma in biopharmaceutical buffer formulations (both upstream and downstream applications), electrophoresis separations and diagnostic reagent formulations.
所有缓冲液的pH值都与温度和浓度有关。对于Tris缓冲液，降温每℃其pH值约增加0.03单位，每10倍稀释其pH降低0.03-0.05单位。
对于精确的应用，请使用带有玻璃/甘汞组合电极并仔细校准过的pH计。
法律信息
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of sigma-aldrich Co. LLC
基本信息
| 线性分子式 | NH2C(CH2OH)3 · HCl |
| 分子量 | 157.60 |
| Beilstein | 3675235 |
| MDL编号 | MFCD00012590 |
产品性质
| 质量水平 | 400 |
| 生物来源 | synthetic |
| 描述 | Manufactured under appropriate controls for use as a raw material in BioProcessing |
| 产品线 | EMPROVE® EVOLVE |
| 形式 | powder |
| technique(s) | cell culture | mammalian: suitable |
| 杂质 | Endotoxin, tested Microbial, tested Trace Metals, tested |
| useful pH range | 7.0-9.0 |
| pKa (25 ℃) | 8.1 |
| 适用性 | suitable for manufacturing use |
| 异质活性 | Cytotoxicity, contains (tested) DNase, contains (tested) NICKase, contains (tested) Protease, contains (tested) RNase, contains (tested) |
| SMILES string | Cl.NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H |
| InChI key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
