产品说明
一般描述
小鼠白血病抑制因子(LIF,mLIF)通过抑制自发分化,促进多能小鼠胚胎干(ES)和诱导多能干(iPS)细胞的自我更新和长期维持。
ESGRO 小鼠 LIF 培养基补充剂以有效单位/mL 的形式提供,以确保批次之间的性能一致。 每批 ESGRO 补充剂均经过严格的质量控制测试,以确保能可靠地抑制小鼠多能干细胞分化。 ESGRO 小鼠 LIF 补充剂可用于小鼠胚胎干(ES)和诱导性多能干(iPS)细胞的基于饲养层和无饲养层的培养。
应用
ESGRO
建议的实验方案:
ES 细胞:在 D3 和 MBL-1 多能 ES 细胞培养物中,研究发现,通常每 1.0 mL 组织培养基需要 1000 U ESGRO
在推荐浓度下,107 单位的 ESGRO
外形
ESGRO
形式:纯化
制备说明
提供的 ESGRO® 补充剂经 0.22 微米无菌过滤,并在支原体检测中呈阴性。
储存及稳定性
ESGRO
分析说明
比活度:ESGRO® 补充剂在小鼠胚胎干细胞(D3 & MBL-1)和鼠 M1 髓样白血病细胞上进行了评估(4)。50 单位的标准定义为在 1.0mL 组织培养基中可诱导 50% 的 M1 菌落分化的 ESGRO
胚胎干细胞测定:分化抑制测定,浓度为 1000 单位/mL 小鼠骨髓白血病细胞 M1:比活度 >/= 108 单位/mg
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
基本信息
eCl@ss | 32160801 |
NACRES | NA.75 |
产品性质
质量水平 | 100 |
测定 | >95% (active component mLIF, SDS-PAGE) |
形式 | liquid |
manufacturer/tradename | Chemicon® |
浓度 | 10000000 units/mL |
technique(s) | cell culture | stem cell: suitable |
input | sample type induced pluripotent stem cell(s) sample type: mouse embryonic stem cell(s) |
NCBI登记号 | NM_008501 |
UniProt登记号 | P09056 |
运输 | wet ice |
Gene Information | mouse ... LIF(16878) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |