位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Tumor Necrosis Factor-α Antibody, clone 2C8

品牌
Sigma-Aldrich
货号
MAB1021
规格纯度
clone 2C8, Chemicon®, from mouse
参考价格
4513.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.

免疫原

Human recombinant tumor necrosis factor α.

应用

Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.

Immunohistochemistry detected TNF-α.

TNF-α Neutralization Assay

1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.

2. Incubate overnight at 37°C in humidified 5% CO2 incubator.

3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL

4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.

5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.

6. Incubate 24 hours at 37°C, 5% CO2.

7. Score wells for cytotoxicity.

Optimal working dilutions must be determined by the end user.

联系

Replaces: 04-1114

外形

Protein A purified
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to one year.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified antibody
克隆2C8, monoclonal
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable
同位素/亚型IgG1
NCBI登记号NM_000594.2
UniProt登记号P01375
运输wet ice
Gene Informationhuman ... TNF(7124)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号