产品说明
特异性
Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.
免疫原
Human recombinant tumor necrosis factor α.
应用
Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
联系
Replaces: 04-1114
外形
Protein A purified
Format: Purified
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for up to one year.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
克隆 | 2C8, monoclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable neutralization: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_000594.2 |
UniProt登记号 | P01375 |
运输 | wet ice |
Gene Information | human ... TNF(7124) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |