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Anti-phospho-RNAPII Antibody (Thr4), clone 1G7

品牌
Sigma-Aldrich
货号
MABE348
规格纯度
clone 1G7, from rat
参考价格
5854.74 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

特异性

This antibody recognizes RNAPII phosphorylated at Thr4.

免疫原

Ovalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Thr4.
Epitope: Phosphorylated Thr4

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cell extracts (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Thr4) in HeLa cells (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Thr4) in stably transfected Raji cell lysates (Hintermair, C., et al. (2012). EMBO J. 31(12):2784-2791.).
Research Category
Epigenetics & Nuclear Function
Detect the phospho-RNAPII (Thr4) protein using this Anti-phospho-RNAPII (Thr4), clone 1G7 validated for use in wb, IP, ICC & ChIP.

质量

Evaluated by Western Blot in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: A 1:500 dilution of this antibody detected phospho-RNAPII (Thr4) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)

目标描述

~220 kDa observed

外形

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Untreated and lambda phosphatase treated HeLa cell lysates.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源rat
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1G7, monoclonal
species reactivityyeast, human, mouse
technique(s)ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_000928
UniProt登记号P24928
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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