产品说明
一般描述
Monoclonal Anti-JAK2 (mouse IgG1 isotype) is derived from the hybridoma 10.1.5 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Janus kinases (Jak) family of proteins includes four members: Jak1, Jak2, Jak3 and the tyrosine kinase 2 (Tyk2). Jak2 is ubiquitously expressed. It is also expressed in normal and neoplastic mammary epithelial cells.
免疫原
synthetic peptide corresponding to an internal sequence of human JAK2 (GeneID 3717), conjugated to KLH.
应用
Monoclonal Anti-JAK2 antibody produced in mouse has been used in:
- immunoblotting
- immunocytochemistry
- flow cytometry
生化/生理作用
Janus kinases engage in interactions with the intracellular tails of several cytokine receptors, which result in the activation of members of the signal transducer and activator of transcription (STAT) family of transcription factors. The latter bind specific cognate sequences at the promoter region of genes that regulate proliferation and differentiation. JAK activation plays an important role in cell proliferation, differentiation, migration and apoptosis. A member of this family, JAK2, has been found to induce signaling regulating gene expression of various pro-survival and anti-apoptotic molecules. Furthermore, inhibition of JAK2 signaling triggered cell death in human cancer cells. These findings indicate JAK2 inhibition as a potential therapeutic approach for various cancers.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.44 |
产品性质
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | 10.1.5, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~125 kDa |
| species reactivity | bovine, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using MCF7 cells. western blot: 2.0-4.0 μg/mL using TF1 total cell extracts. |
| UniProt登记号 | O60674 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... JAK2(3717) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
