产品说明
特异性
Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.
(original paper claimed IgG2ak)
免疫原
Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.
应用
Research Category
Cell Structure
Immunoprecipitation
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
Research Sub Category
Integrins
This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.
联系
Replaces: 04-1060
外形
In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.
Format: Purified
储存及稳定性
Maintain between 2 and 8°C.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 25E11, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable immunoprecipitation (IP): suitable |
| 同位素/亚型 | IgM |
| NCBI登记号 | NM_000212.2 |
| UniProt登记号 | P05106 |
| 运输 | wet ice |
| Gene Information | human ... ITGB3(3690) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
