位置:首页 > 品牌 > Sigma-Aldrich >

Anti-GATA Antibody3, clone 7B5

品牌
Sigma-Aldrich
货号
MABD117
规格纯度
ascites fluid, clone 7B5, from mouse
参考价格
5688.25 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

GATA-binding factor 3 (GATA3), also known as Trans-acting T-cell-specific transcription factor GATA-3, is a transcription factor containing a zinc finger domain. GATA3 is primarily expressed in T cells, where it thought to participate in T-cell receptor gene activation through binding to enhancers. Analysis of conditional GATA3 knockout mice confirmed the critical role of GATA3 in helper T cell differentiation and proliferation. Cells expressing GATA3 develop the profile of Th2 cells, secreting IL-4, IL-5 and IL-13. Binding of GATA3 in the regulatory regions of the genes of these cytokines alters chromatin structure, increasing accessibility to other transcription factors. T cell receptor activation activates NFAT and other transcription factors that cooperate with GATA3 in inducing Th2 cell differentiation. Blocking GATA3 action has been suggested as a therapeutic strategy to treat asthma. GATA3 has also been implicated in developmental processes such as the development of the inner ear.

免疫原

Purified recombinant fragment of human GATA3 expressed in E. Coli.

应用

Detect GATA using this mouse monoclonal antibody, Anti-GATA Antibody3, clone 7B5 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA3 in human small intestine tissue.

Optimal working dilutions must be determined by end user.


Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research

质量

Evaluated by Western Blotting in GATA3-hIgGFc transfected HEK293 cell lysate.

Western Blot Analysis: A 1:500-2000 dilution of this antibody detected GATA3 in GATA3-hIgGFc transfected HEK293 cell lysate.

目标描述

~75 kDa observed

外形

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
GATA3-hIgGFc transfected HEK293 cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆7B5, monoclonal
species reactivityhuman
technique(s)immunohistochemistry: suitable
western blot: suitable
同位素/亚型IgG1
UniProt登记号P23771
运输wet ice
Gene Informationhuman ... GATA3(2625)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号