产品说明
特异性
Go-alpha. Strong reactivity with bovine (100%), human (70%), rat (61%) and mouse (54%). Slight reactivity with guinea pig (27%). Specificity confirmed by Western blot versus bovine G-alpha-t, rat Go-alpha, rat Gi-alpha-1, Gi-alpha-2, rat Gi-alpha-3, bovine Gs-alpha, and murine Go-alpha-A and Go-alpha-B. The carboxy-terminal splice variants of Go-alpha (Go-alpha-A and Go-alpha-B) expressed in bacterial lysates were compared for reactivity and found to be equal . As Go-alpha-A and Go-alpha-B have >95% homology to rat Go-alpha-1 and human Go-alpha-2, MAB3073 will detect these G-proteins also.
免疫原
Bovine brain Go-alpha purified by the method of Sternweis and Robishaw (J. Biol. Chem. [1984] 259, 13806).
应用
Research Category
Signaling
Anti-G Protein Goα Antibody, clone 2A is an antibody against G Protein Goα for use in WB.
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
Western blot 1:3000.
Labels a single protein band of 39 - 42 kDa in bovine and rat membranes.
Optimal working dilutions must be determined by end user.
外形
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl, pH 7.6, containing 0.1% sodium azide as a preservative.
Format: Purified
储存及稳定性
Maintain at 2-8°C in undiluted aliquots for upt to 6 months after date of receipt.
During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 2A, monoclonal |
| species reactivity | guinea pig, human, bovine, rat, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG1 |
| NCBI登记号 | NM_020988.1 NM_138736.1 |
| UniProt登记号 | P29777 |
| 运输 | wet ice |
| Gene Information | human ... GNAO1(2775) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
