Anti-FOXK2 Antibody

产品说明

一般描述

Forkhead box protein K2 (UniProt Q01167; also known as FOXK2, Cellular transcription factor ILF-1, Interleukin enhancer-binding factor 1) is encoded by the FOXK2 (also known as ILF, ILF-1, ILF1) gene (Gene ID 3607) in human. FOXK2 is a transcription factor involved in cell proliferation and survival. FOXK1 and FOXK2 belong to the family of Fox transcription factors characterized by the forkhead DNA-binding domains. However, FOXK1 and FOXK2 can be distinguished from other Fox transcription factors by the presence of the forkhead-associated (FHA) domains. FOXK2 interacts with BRCA1-associated protein 1 (BAP1) through the FHA domain in a BAP1 Thr493 phosphorylation-dependent manner. BAP1 in turn recruits HCF-1, thereby forming a ternary complex at the FOXK2 target genes sites, where BAP1 represses FOXK2 target genes expression in a BAP1 DUB activity-dependent manner.

特异性

Expected to react with isoforms 1&2, but not isoform 3.

免疫原

Epitope: C-terminal
Recombinant protein corresponding to the C-terminal region of human FOXK2.

应用

Immunoprecipitation Analysis: A representative lot immunoprecipitated FOXK2 in HeLa nuclear extract (Data courtesy of El Bachir Affar, University of Montreal).
Western Blotting Analysis: A representative lot detected FOXK2 in tag-affinity purified BAP1 complex from HeLa cells expressing Flag-HA-tagged wild-type BAP1 or catalytically dead C91S BAP1 mutant (Mashtalir, N., et al. (2014). Mol. Cell. 54(3):392-406).
Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
This Anti-FOXK2 Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of FOXK2 .

质量

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FOXK2 in 10 µg of HeLa nuclear extract.

目标描述

~78 kDa observed. Isoform 1 (69.1 kDa calculated); Isoform 2 (64.3 kDa calculated).

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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