产品说明
特异性
Recognizes Human collagen type I. Some cross reactivity with other proteins.
免疫原
Human placental collagen type I
应用
ELISA on human collagen type I: 1:4,000.
Indirect microimmunofluorescent visualization of collagen type I on cryostat sections of human tissue or cultured cells: 1:10-1:40.
Western blot 1:200
Not recommended for use in paraffin treated tissues.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
This Anti-Collagen Type I Antibody is validated for use in ELISA, IH, WB for the detection of Collagen Type I.
Research Sub Category
ECM Proteins
外形
Format: Semi-Purified
Immunoglobulin fraction, prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiSerum cross-absorbed over immobilized human albumin, immunoglobulin and collagen types III, IV and V.
Product is liquid in 0.15M Sodium Chloride, 10mM Sodium Phosphate pH 7.5 with 0.1% Mannitol and 0.1% Dextran as stabilizers. No preservative.
Ammonium sulfater precipitation followed by DEAE chromatography, and immunodeletion
储存及稳定性
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin (Semi-purified) |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
manufacturer/tradename | Chemicon® |
technique(s) | ELISA: suitable immunohistochemistry: suitable western blot: suitable |
适用性 | not suitable for immunohistochemistry (Paraffin) |
NCBI登记号 | NP_000079 |
UniProt登记号 | P02452 |
运输 | dry ice |
Gene Information | human ... COL1A1(1277) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |