产品说明
特异性
MAB3507 antibody recognizes a reactive epitope that lies in the P19 subunit of human Caspase-2 (ICH-1, Nedd-2).
免疫原
Raised against the P19 subunit of recombinant human Caspase-2 protein.
应用
Western blot: Recommended usage at 5 μg/ml, which has been found to detect endogenous Caspase-2 in 5 x 10E5 HeLa and MCF-7 cells.
Immunoprecipitation: Recommended concentration 1-5 μg/ml FACS: Recommended concentration 1 μg/ml. It is necessary to permeabilize the cell membrane prior to analysis.
EIA: Recommended concentration 0.5-1 μg/ml.
Immunohistochemistry: Use at 10-20 μg/ml. It is necessary to fix the cells in 4% Paraformaldehyde/0.18% Triton-X100 for 10 minutes prior to staining.
Optimal working dilutions must be determined by end user.
Anti-Caspase 2 Antibody, clone 11B4 is an antibody against Caspase 2 for use in ELISA, FC, IP & WB.
Research Sub Category
Caspases
Enzymes & Biochemistry
Research Category
Apoptosis & Cancer
Metabolism
联系
Replaces: 04-571
外形
Liquid with 0.05% Bronidox as a preservative.
Format: Purified
储存及稳定性
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | rat |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 11B4, monoclonal |
| species reactivity | canine, human, mouse, monkey |
| manufacturer/tradename | Chemicon® |
| technique(s) | ELISA: suitable flow cytometry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG2a |
| NCBI登记号 | NM_032982.2 NM_032983.2 |
| UniProt登记号 | P42575 |
| 运输 | wet ice |
| Gene Information | human ... CASP2(835) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
