产品说明
一般描述
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
特异性
Expected to cross-react with porcine, chicken, bovine, and rabbit.
The antibody reacts with GFAP from human, pig, chicken and rat. In tissue sections this antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).
免疫原
Purified glial filament (Debus, E., 1983).
应用
Research Sub Category
Neuronal & Glial Markers
Immunocytochemistry:
5 μg/mL of a previous lot was used.
Immunohistochemistry:
5 µg/mL
Immunoblotting:
Recognizes a 51kDa protein in reducing westerns of total brain lysates.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 is an antibody against Glial Fibrillary Acidic Protein for use in IC, IH, IH(P) & WB with more than 55 product citations.
质量
Routinely evaluated by Western Blot on Mouse brain lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Glial Fibrillary Acidic Protein on 10 μg of Mouse Brain lysate.
目标描述
50 kDa
联系
Replaces: 04-1031; 04-1062
外形
Purified mouse monoclonal IgG1 in buffer containing 0.02 M phosphate buffer, 0.25 M NaCl with 0.1% sodium azide, pH 7.6.
Format: Purified
Protein A purified
储存及稳定性
Store the reconstituted antibody at 2-8°C for up to 6 months after date of receipt.
DO NOT FREEZE.
分析说明
Control
Mouse brain tissue, Astrocyte culture.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | GA5, monoclonal |
species reactivity | chicken, rat, rabbit, mouse, pig, bovine, human |
manufacturer/tradename | Chemicon® |
technique(s) | immunocytochemistry: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_002055.2 |
UniProt登记号 | P14136 |
运输 | wet ice |
Gene Information | human ... GFAP(2670) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |