产品说明
一般描述
c-Fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-Fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
特异性
This antibody recognizes the N-terminus of c-Fos.
免疫原
KLH-conjugated linear peptide corresponding to the N-terminus of human c-Fos.
应用
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Immunofluorescence Analysis: A representative lot detected c-Fos in rat pons and rat cerebellum tissues.
Anti-c-Fos Antibody is a Rabbit Polyclonal Antibody for detection of c-Fos also known as Proto-oncogene c-Fos, Cellular oncogene fos & has been validated in WB & IHC.
质量
Evaluated by Western Blot in PMA(TPA) treated HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected c-Fos in 10 µg of PMA(TPA) treated HeLa cell lysate.
目标描述
~60/56 kDa observed. This protein has multiple isoforms produced by alternative splicing ranging between 53 and 68 kDa (Jurado, J., et al. (2007). BMC Molecular Biology. 8:83-96.)
外形
Affinity purified
分析说明
Control
PMA(TPA) treated HeLa cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, rat |
| species reactivity (predicted by homology) | bovine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), porcine (based on 100% sequence homology), mouse (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), sheep (based on 100% sequence homology) |
| technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
| NCBI登记号 | NP_005243 |
| UniProt登记号 | P01100 |
| 运输 | wet ice |
| Gene Information | human ... FOS(2353) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
