产品说明
一般描述
c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.
特异性
Recognizes c-fos.
免疫原
Synthetic c-fos peptide (residues Ser.Gly.Phe.Asn.Ala.Asp.Tyr.Glu.Ala. Ser.Ser.Ser.Arg.Cys) corresponding to amino acids 4-17 of human Fos, conjugated to bovine thyroglobulin.
应用
Research Sub Category
Transcription Factors
Research Category
Epigenetics & Nuclear Function
Immunohistochemistry: 1:500-1:4000, 2-8°C 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Recommended fixation is 4% PFA with 0.5% picric acid {Li et al 1999}; triton X-100 pretreatment in the block only; antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.
Western blot: 10 μg/mL. Detects a band at approximately 55 kDa.
Optimal working dilutions must be determined by the end user.
Use Anti-c-Fos Antibody (Sheep Polyclonal Antibody) validated in IHC, IHC(P), WB to detect c-Fos also known as G0/G1 Switch Regulatory Protein 7.
目标描述
55 kDa
外形
Purified immunoglobulin. Lyophilized, no preservatives. Reconstitute with 500 μL of sterile distilled water (1 mg/mL).
Format: Purified
Ammonium Sulfate Precipitation
储存及稳定性
Maintain lyophilized material at -20 to -70°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to one year.
Avoid repeated freeze/thaw cycles.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.
分析说明
Control
Widely expressed
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
质量水平 | 100 |
生物来源 | sheep |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | rat |
manufacturer/tradename | Chemicon® |
technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable western blot: suitable |
NCBI登记号 | NM_005252.2 |
UniProt登记号 | P01100 |
运输 | wet ice |
Gene Information | human ... FOS(2353) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |