产品说明
一般描述
Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. The pharmacophore of imidazole exists in bioactive compounds including amino acids, plant growth regulators and therapeutic agents.
应用
Imidazole has been used
- in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
- in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
- as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
特别适用于 pH 6.2-7.8 范围内的缓冲液
生化/生理作用
Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).
法律信息
Redi-Dri is a trademark of sigma-aldrich Co. LLC
基本信息
| 经验(实验)分子式 | C3H4N2 |
| 分子量 | 68.08 |
| Beilstein | 103853 |
| MDL编号 | MFCD00005183 |
产品性质
| 质量水平 | 100 |
| 等级 | for molecular biology |
| 蒸汽压 | <1 mmHg ( 20 ℃) |
| 产品线 | Redi-Dri™ |
| 测定 | ≥99% (titration) |
| 形式 | crystalline powder |
| 质量 | free-flowing |
| pH值(酸碱度) | 6.2-7.8 (25 ℃) |
| pKa (25 ℃) | 6.95 |
| bp | 256 ℃ (lit.) |
| mp | 88-91 ℃ (lit.) |
| application(s) | diagnostic assay manufacturing |
| 异质活性 | DNase, RNase and protease, none detected |
| SMILES string | c1c[nH]cn1 |
| InChI | 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5) |
| InChI key | RAXXELZNTBOGNW-UHFFFAOYSA-N |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H302 - H314 - H360D |
| 预防措施声明 | P201 - P260 - P280 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313 |
| 危险分类 | Acute Tox. 4 Oral - Eye Dam. 1 - Repr. 1B - Skin Corr. 1C |
| 储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |
| WGK | WGK 2 |
| 闪点(F) | 293.0 °F - closed cup |
| 闪点(C) | 145 ℃ - closed cup |
