产品说明
一般描述
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
特异性
Clone LTF-2 is an isotype control antibody for rat IgG2b.
应用
Quality Control Testing:
Evaluated by Flow Cytometry in Protein A/G beads.
Rat IgG2b Isotype Control (LTF-2) ColorWheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.
相容性
Requires ColorWheel® Dye (Sold Separately)
目标描述
Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rat IgG2b Isotype Control, clone LTF-2 is suitable for use as an isotype-matched control for rat IgG2b. It has an unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a rat IgG2b and the sample under defined experimental conditions.
外形
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
重悬
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
储存及稳定性
Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
法律信息
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 质量水平 | 200 |
| 生物来源 | rat |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | LTF-2, monoclonal |
| 产品线 | ColorWheel® |
| 形式 | lyophilized |
| species reactivity | rat |
| 包装 | antibody small pack of 25 μL |
| technique(s) | flow cytometry: suitable |
| 同位素/亚型 | IgG2b |
| 相容性 | for use with ColorWheel® Dyes (Required, sold separately) |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
