产品说明
一般描述
Product Source: GST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1, expressed in E. coli.
质量
Routinely evaluated by G-protein activity assay
外形
300 µg of PAK-1 PBD, GST in 150 µL of PBS, pH 7.4, 0.5 mM PMSF, containing 20% glycerol.
GSH-agarose chromatography
储存及稳定性
Stable for 1 year at -20C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
分析说明
Specific Activity: Affinity Precipitation Assay: 10 µg of this lot when added with 10 µL glutathione agarose, precipitated Rac-GTP from 3T3 and A431 cell lysates that were loaded in vitro with GTPγS. GDP treated of 3T3 and A431 lysates served as negative controls. Precipitated Rac-GTP was detected by immunoblot analysis using 1 &micor;g/mL anti-Rac (Catalog # 05-389) and secondary goat anti-mouse HRP antibody (Catalog # 12-349) on a Western Blot.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160405 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human |
| 测定 | >50% (Coomassie blue staining, SDS-PAGE) |
| 分子量 | Mw 21 kDa |
| manufacturer/tradename | Upstate® |
| 浓度 | 2 mg/mL |
| technique(s) | ELISA: suitable affinity binding assay: suitable (G-protein) |
| NCBI登记号 | NM_002576.3 |
| UniProt登记号 | Q13153 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
