产品说明
一般描述
Phosphorylation of the histone variant H2A.X is a rapid and sensitive response to double strand DNA breaks. The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated histone H2A.X.
The H2A.X Phosphorylation Assay Kit (Flow cytometry) is a cell based assay formatted for flow cytometric detection of levels of phosphorylated Histone H2A.X. Cells are cultured in microplates, treated with agents that induce DNA damage or apoptosis, which stimulates H2A.X phosphorylation. Cells are then fixed and permeabilized in preparation for staining and detection. Histone H2A.X phosphorylated at serine 139 is detected by the addition of the anti-phospho-Histone H2A.X, FITC conjugate. Cells are then scanned in a flow cytometer to quantitate the number of cells staining positive for phosphorylated Histone H2A.X.
应用
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
包装
100 assays
组分
Anti-phospho H2A.X, FITC conjugate
Normal Mouse IgG, FITC conjugate (Cat.# 12-487)
16X Fixation Solution
10X Permeabilization Solution
10X Wash Solution
质量
phosphorylation of Histone H2A.X
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32161000 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| manufacturer/tradename | Upstate® |
| technique(s) | activity assay: suitable flow cytometry: suitable |
| NCBI登记号 | NM_002105.2 |
| UniProt登记号 | P16104 |
| detection method | fluorometric |
| 运输 | dry ice |
安全信息
| 象形图 |     |
| 警示用语： | Danger |
| 危险声明 | H225 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370 |
| 预防措施声明 | P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 3 - Flammable liquids |
| WGK | WGK 3 |
