产品说明
一般描述
Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate
Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
特异性
Tyrosine-phosphorylated proteins from all species.
免疫原
Phosphotyramine-KLH
应用
Research Sub Category
General Post-translation Modification
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10
Research Category
Signaling
质量
Routinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
目标描述
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
外形
Biotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
Protein G Purified
储存及稳定性
Stable for 9 months at 2-8ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
分析说明
Control
A431 carcinoma cells.
Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | biotin conjugate |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 4G10®, monoclonal |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Upstate® |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
