产品说明
一般描述
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.
Description
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10
Anti-Phosphotyrosine antibody (05-321), cross-linked to horseradish peroxidase
特异性
Recognizes tyrosine-phosphorylated proteins from all species.
免疫原
Phosphotyramine-KLH.
应用
Anti-Phosphotyrosine Antibody, clone 4G10
For use in applications in which an HRP conjugate is advantageous, such as WB and IP.
Research Sub Category
General Post-translation Modification
Research Category
Signaling
质量
Routinely evaluated in western blot on RIPA lysates from EGF-treated human A431 carcinoma cells.
Western Blot Analysis:
Tested at 0.5-2 μg/mL, with optimal performance at 2 μg/mL (10 μL per 10 mL blocking buffer). This lot detected tyrosine-phosphorylated proteins in RIPA lysates from EGF-treated human A431 carcinoma cells.
目标描述
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
外形
200 μg of IgG2bκ-HRP conjugate in 100 μL PBS containing 0.05% Kathon® . Sufficient for 10 western blots, if used at the optimum concentration of 2 μg/mL in 10 mL blocking buffer. Liquid.
Protein G-Sepharose
储存及稳定性
Stable for 6 months at 2-8°C from date of receipt. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
NOTE: For optimal performance, remove HRP precipitate by centrifuging vial prior to use.
分析说明
Control
Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
法律信息
4G10 is a registered trademark of Upstate Group, Inc.
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.56 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | peroxidase conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 4G10®, monoclonal |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Upstate® |
| technique(s) | ELISA: suitable western blot: suitable |
| 同位素/亚型 | IgG2bκ |
| 运输 | wet ice |
安全信息
| 象形图 |   |
| 警示用语： | Warning |
| 危险声明 | H317 - H411 |
| 预防措施声明 | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
| 危险分类 | Aquatic Chronic 2 - Skin Sens. 1 |
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
