Anti-Mouse CD23 (2G8) ColorWheel^® Dye-Ready mAb

产品说明

一般描述

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

特异性

Clone 2G8 is a rat monoclonal antibody that detects murine CD23.

免疫原

FceR isolated from murine B cell lymphoma cell line M12.4.5

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Mouse splenocytes.

Flow Cytometry Analysis: Staining of one million mouse splenocytes was performed using 5 μL of 1:1 mixture of Cat. No. CWA-1003, Anti-Human CD23 (2G8) ColorWheel^® Dye-Ready mAb and CWD-PE, ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of a PE-conjugated Rat IgG2a isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel^® Dye (Sold Separately)

目标描述

Low affinity immunoglobulin epsilon Fc receptor (UniProt: P20693; also known as Fc-epsilon-RII, Lymphocyte IgE receptor, CD23) is encoded by the Fcer2 (also known as Fcr2a) gene (Gene ID: 14128) in murine species. CD23 is a homotrimeric, single-pass type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-23); a transmembrane domain (aa 24-49), and an extracellular domain (aa 50-331). The extracellular domain presents striking similarity to C-type lectins. CD23 expression is reported in B cells, monocyte, follicular dendritic cells, platelets, and eosinophils. It is also expressed in cases of chronic lymphocytic leukemia (CLL) and in follicular lymphomas. It serves as a low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in B-cell differentiation. In addition to its role as a receptor for IgE, CD23 can also be released from cell surface as a soluble protein (sCD23) via ADAM10-mediated ectoderm shedding. Mice lacking ADAM10 expression in their B cells display defective release of CD23. Clone 2G8 is reported to interact at a site similar to but not identical with the site on CD23 that binds IgE. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460; Margaret, E., et al. (1999). J. Immunol. 163(1); 217-223; Acharya, M., et al. (2010). Clin. Exp. Immunol. 162(1); 12-23).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

安全信息