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Anti-Human CD69 (FN50) Colorwheel® Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1037
规格纯度
for use with ColorWheel® Dyes (Required, sold separately)
参考价格
1596.49 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

特异性

Clone FN50 is a mouse monoclonal antibody that detects human CD69.

免疫原

Anti-μ stimulated human B lymphocytes.

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) stimulated with PMA/Ionomycin.

Staining of one million human peripheral blood mononuclear cells (PBMC) stimulated with Ionomycin/PMA (1 μg/mL, 4 h) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1037, Anti-Human CD69 (FN50) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel® Dye (Sold Separately)

目标描述

Early activation antigen CD69 (UniProt: Q07108; Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69) is encoded by the CD69 (also known as CLEC2C) gene (Gene ID: 969) in human. CD69 is a single-pass type II membrane disulfide-linked homodimeric protein with a cytoplasmic domain (aa 1-40), a transmembrane domain (aa 41-61), and an extracellular domain (aa 62-199). It is a C-type lectin and a member of the natural killer (NK) receptor family. It is an early activation marker that is expressed in hematopoietic stem cells, activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets. It is induced by antigens, mitogens, or activators of PKC on the surface of T and B-lymphocytes and by interaction of IL-2 with the p75 IL-2R on the surface of NK cells. CD69 is involved in immune cell homeostasis and regulates T cell-mediated immune response through the control of Th17 cell differentiation. Expression of CD69 by activated T lymphocytes is reported to trigger an anti-inflammatory mechanism mediated by Galectin-1, which regulates the immune response and prevents pathogenic Th17 responses. CD69 knockout mice are shown to develop an exacerbated form of collagen-induced arthritis. (Ref.: de la Fuente, H., et al. (2014). Mol. Cell. Biol. 34(13); 2479-2487; Ziegler SF et al., (1994). Stem Cells. 12(5); 456-65).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆FN50, monoclonal
产品线ColorWheel®
形式lyophilized
分子量calculated mol wt 22.56 kDa
species reactivityhuman
包装antibody small pack of 25 μL
technique(s)flow cytometry: suitable
同位素/亚型IgG1
表位序列Unknown
Protein ID登记号CAA80298.1
UniProt登记号Q07108
相容性for use with ColorWheel® Dyes (Required, sold separately)
运输ambient
储存温度2-8℃
Gene Informationhuman ... CD69;CLEC2C(969)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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