位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Human CD64 (10.1) Colorwheel® Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1026
规格纯度
for use with ColorWheel® Dyes (Required, sold separately)
参考价格
1596.49 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

特异性

Clone 10.1 is a mouse monoclonal antibody that detects CD64 (FCgR1). It targets an epitope within the extracellular domain.

免疫原

Human rheumatoid synovial fluid cells and fibronectin purified monocytes.

应用

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1026, Anti-Human CD54 (10.1) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel® Dye (Sold Separately)

目标描述

High affinity immunoglobulin gamma Fc receptor I (UniProt: P12314; also known as IgG Fc receptor I, Fc-gamma RI, FcRI, Fc-gamma RIA, FcgammaRIa, CD64) is encoded by the FCGR1A (also known as FCG1, FCGR1, IGFR1) gene (Gene ID: 2209) in human. CD64 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-15), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 16-292), a transmembrane domain (aa 293-313), and a cytoplasmic domain (aa 314-374). Within its extracellular domain are three Ig-like C2 type domains. Its expression has been reported in monocytes/macrophages. Expression of CD64 distinguishes conventional dendritic cells from monocyte-derived dendritic cells. It serves as a high-affinity receptor for the Fc domains of IgG (FcR) and plays a key role in linking the humoral and the cellular arms for the immune system. It binds human IgG1, IgG3, and IgG4 with a high affinity, but does not show any affinity for IgG2. It functions in both innate and adaptive immune responses. Along with the low affinity receptor (FcRII), it mediates a variety of biological responses, including endocytosis, phagocytosis, antibody-directed cellular cytotoxicity, and cytokine release. Clone 10.1 is shown to react with an epitope on FcRI near to the binding site for the Fc region of IgG. (Ref.: Harrison, PT., and Allen., (1998). Protein Engineering. 11 (3); 225-232; Dougherty, GJ., et al. (1987). Eur. J. Immunol. 17(10); 1453-1459).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆10.1, monoclonal
产品线ColorWheel®
形式lyophilized
分子量calculated mol wt 42.63 kDa
species reactivityhuman
包装antibody small pack of 25 μL
technique(s)flow cytometry: suitable
同位素/亚型IgG1κ
表位序列Extracellular domain
Protein ID登记号NP_001365740.1
UniProt登记号P12314
相容性for use with ColorWheel® Dyes (Required, sold separately)
运输ambient
储存温度2-8℃
Gene Informationmouse ... FCGR1A(2209)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号