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Anti-Human CD56 (MY31) Colorwheel® Dye-Ready mAb

品牌
Sigma-Aldrich
货号
CWA-1067
规格纯度
for use with ColorWheel® Dyes (Required, sold separately)
参考价格
1596.49 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

特异性

Clone MY31 is a mouse monoclonal antibody that detects Neural cell adhesion molecule 1 (CD56).

免疫原

KG1a leukemia cells

应用

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1067, Anti-Human CD56 (MY31) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Evaluated by Flow Cytometry in Human NK cells.

Staining of one million Human NK cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1067, Anti-Human CD56 (MY31) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye (yellow histogram) or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

相容性

Requires ColorWheel® Dye (Sold Separately)

目标描述

Neural cell adhesion molecule 1 (UniProt: P13591; also known as N-CAM-1, NCAM-1, CD56) is encoded by the NCAM1 (also known as NCAM) gene (Gene ID: 4684) in human. CD56 is a member of the immunoglobulin superfamily that is engaged in both so-called homophilic and heterophilic interactions. It is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-718), a transmembrane domain (aa 719-739), and a cytoplasmic domain (aa 740-858). It also contains five Ig-like C2-type domains and two fibronectin type-III domains. CD56 is expressed by neurons, glia, skeletal muscle, and natural killer (NK) cells. It is involved in neuron-neuron adhesion, neurite fasciculation, and outgrowth of neurite. It is considered as the archetypal phenotypic marker for natural killer cells, but is also expressed in many other immune cells, including ab T cells, g T cells, dendritic cells, and monocytes. It is also shown to play a role in the fungus mediated NK cell activation and blocking of CD56 surface receptor reduced fungal mediated NK cell activation and reduced cytokine secretion. (Ref.: Ziegler, S., et al. (2017). Sci. Rep. 7; Article 6138; Van Acker, HH., et al. (2017). Front. Immunol. 8; Article 892).

外形

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

重悬

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

法律信息

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

质量水平200
生物来源mouse
抗体形式purified antibody
antibody product typeprimary antibodies
克隆MY31, monoclonal
产品线ColorWheel®
形式lyophilized
分子量calculated mol wt 94.57 kDa
species reactivityhuman
species reactivity (predicted by homology)nonhuman primates
包装antibody small pack of 25 μL
technique(s)flow cytometry: suitable
同位素/亚型IgG1κ
表位序列Unknown
Protein ID登记号NP_000606.3
UniProt登记号P13591
相容性for use with ColorWheel® Dyes (Required, sold separately)
运输ambient
储存温度2-8℃
Gene Informationhuman ... NCAM1(4684)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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