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Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free

Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free

品牌
Sigma-Aldrich
货号
MAB2019Z
规格纯度
clone 15F11, Chemicon®, from mouse
参考价格
5426.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Reactive with human integrin alphaVbeta5. Does not recognize integrin alphaVbeta3 or alpha5beta1. The antibody binds to the alphaVbeta5 complex. The monoclonal antibody MAB2019Z has a Kd of 2.8 nM in Mn2+, 4.0 nM in Ca2+and 2.9 nM in buffer lacking cation. Thus, cations do not significantly affect the binding of the antibody to alphaVbeta5. Monoclonal antibody MAB2019Z does not block ligand binding to purified alphaVbeta5, nor does it block cell adhesion mediated through this receptor.

免疫原

Generated against human integrin alphaVbeta5 purified from placental tissue and depleted of integrin alphaVbeta3.

应用

Research Category
Cell Structure
Research Sub Category
Integrins
Immunoprecipitation

Flow Cytometry: MG-63 cells have been harvested, blocked for 20 minutes at 4C in 20% normal human serum then washing in medium containing 10% FBS. 5X10E5 cells per tube were subsequently incubated with primary antibody for 30 min on ice, washed with medium, and incubated with fluorescent-conjugated secondary antibody.

Immunocytochemistry: 15μg/mL dilution for immunostaining of cells adhering to vitronectin-coated coverslips and fixed in 1% paraformaldehyde containing 0.5% Triton X-100 for 20 minutes. Incubation with MAB2019Z antibody for 30 minutes at 22C, followed by detection with a fluorescein-conjugated secondary antibody.

ELISA

Optimal working dilutions must be determined by end user.
Anti-Integrin αVβ5 Antibody, clone 15F11, Azide Free is an antibody against Integrin αVβ5 for use in ELISA, FC, IP & IC.

外形

Purified immunoglobulin. Liquid at 1 mg/mL in PBS, containing no preservatives.
Format: Purified

储存及稳定性

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆15F11, monoclonal
species reactivityhuman
manufacturer/tradenameChemicon®
technique(s)ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
NCBI登记号NM_002210.2
UniProt登记号P06756
运输wet ice
Gene Informationhuman ... ITGAV(3685)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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