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Anti-YAP1 (C-terminal) antibody produced in rabbit

Anti-YAP1 (C-terminal) 兔抗

品牌
Sigma-Aldrich
货号
Y4770
规格纯度
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
7271.36 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

YAP1 (Yes-associated protein 1, YAP, YAP2, YAP65) is a modular adaptor protein with multiple protein interaction domains, that is based on its interaction with Src-family tyrosine kinase c-Yes. It has a SH3-binding motif, proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus. YAP1 also contains a 14-3-3 interacting motif.

特异性

Anti-YAP1 (C-terminal) antibody is specific for human YAP1. The product is expected to react with mouse, bovine, and dog YAP1. In immunoblotting, detection of the YAP1 band is specifically inhibited with the immunizing peptide.

免疫原

synthetic peptide corresponding to amino acids 442-454 located at the C-terminus of human YAP1. The sequence is identical in mouse, bovine, and dog YAP1.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Western Blotting (1 paper)
Anti-YAP1 (C-terminal) antibody is suitable for use in western blot (.5-1 μg/mL) using HEK-293T cells co-transfected with human YAP1 and c-Abl.

生化/生理作用

YAP1 (Yes-associated protein 1) induces p73-dependent apoptosis through the specific and selective co-activation of p53AIP1, an apoptotic p73 target gene. It stabilizes p73 by preventing Itch-mediated ubiquitination of p73. Upon phosphorylation by Akt at Ser127, 14-3-3 is recruited and stimulates YAP1 localization to the cytoplasm, resulting in loss of co-activator function in the nucleus. Inhibition of Akt potentiates the nuclear re-localization of YAP1 to promote apoptosis by p73.
YAP1 is a WW domain containing protein that functions as a transcriptional co-activator . It may also regulate p73 gene function in response to DNA damage. YAP1 is known to associate with a wide range of transcription factors . Alterations in YAP1 have been associated with tumor development .

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD04118707
NACRESNA.44

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
分子量antigen ~65 kDa
species reactivityhuman
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
浓度~1 mg/mL
technique(s)western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and c-Abl
UniProt登记号P46937
运输dry ice
储存温度−20℃
Gene Informationhuman ... YAP1(10413)
mouse ... Yap1(22601)
rat ... Yap1(363014)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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