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Anti-GAD65 Antibody, clone 3B23 ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1091
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2B23 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Glutamate decarboxylase 2 (GAD65). It targets an epitope within 17 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Glutamate decarboxylase 2 (GAD65).

应用

Anti-GAD65, clone 3B23 ZooMAb, Cat. No. ZRB1091, is a recombinant Rabbit monoclonal antibody that targets GAD65 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAD65 human and mouse brain tissue lysates.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAD65 in rat cerebellum tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound human GAD65 peptide with a KD of 1 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:10-100 dilution from a representative lot detected GAD65 in human brain and rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representativelot detected GAD65 in Neuro-2a cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

目标描述

Glutamate decarboxylase 2 (UniProt: Q05329; also known as EC:4.1.1.15, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform) is encoded by the GAD2 (GAD65) gene (Gene ID: 2572) in human. GAD-65 a homodimeric peripheral membrane protein that is associated to cytoplasmic vesicles. It is produced primarily by pancreatic islet cells. It is expressed in human β cells; however, some expression has also been observed in and cells of islets. It is shown to be a major target autoantigen in type 1A diabetes. In neurons, it is present in cytosolic leaflet of Golgi membranes and presynaptic clusters. It is responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions. Two molecular forms of GAD have been described (65 kDa and 67 kDa) that display 64% amino acid homology and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct, and ovary. Between the two isoforms, GAD67 is responsible for over 90% of basal GABA synthesis and is produced at limiting levels in the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit
质量水平200
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆3B23, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 65.41 kDa
observed mol wt ~58 kDa
species reactivityhuman, rat, mouse
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)affinity binding assay: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

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