位置:首页 > 品牌 > Sigma-Aldrich >

Anti-C-EBP alpha Antibody, clone 2E17, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1367
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 2E17 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects C-EBP alpha. It targets an epitope within 147 amino acids from the N-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 147 amino acids from N-terminal region of human C-EBP alpha, isoform 1.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected C-EBP alpha in THP-1 cell lysate.

Flow Cytometry Analysis: 0.01 μg from a representative lot detected C-EBP alpha in one million U937 cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected C-EBP alpha in THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-C-EBP alpha, clone 2E17 ZooMAb, Cat. No. ZRB1367, a recombinant Rabbit monoclonal antibody that specifically targets C-EBP alpha and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

目标描述

CCAAT/enhancer-binding protein alpha (UniProt: P49715; also known as C/EBP alpha, C-EBP alpha) is encoded by the CEBPA (also known as CEBP) gene in human. C-EBP alpha belong to the bZIP family of proteins that serves as a transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. It binds directly to the consensus DNA sequence 5′-T[TG]NNGNAA[TG]-3′ acting as an activator on distinct target genes. It can bind DNA as a homodimer or as a stable heterodimer with CEBPB, CEBPD, CEBPE, and CEBPG. It is reported to be essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP) and has also been shown to be critical for the proper development of the liver and the lung and for terminal adipocyte differentiation. C-EBP alpha down-regulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. It can be phosphorylated on multiple sites. Phosphorylation at serine 190 is shown to be essential for its interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibition. Phosphorylation at threonine 226 and threonine 230 by GSK3 is constitutive in adipose and lung tissue and these sites are also shown to be phosphorylated in liver during feeding, but not during fasting cycle. Four isoforms of C-EBP alpha have been described that are produced by alternative splicing. Mutations in CEBPA gene are known to cause acute myelogenous leukemia (AML). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wedel, A., et al. (1995). Immunobiology. 193(2-4); 171-185; Johnson, PF et al. (2005). J. Cell Sci. 118(12); 2545-2555).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆2E17, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 37.56 kDa
observed mol wt ~50 kDa
species reactivityhuman, mouse
包装pkg of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号